Mat Test For Leptospirosis

The majority of leptospirosis cases are identified through mat serology.

Mat test for leptospirosis.

Detection of leptospiral nucleic acid from a clinical specimen. The microscopic agglutination test mat is the reference test method for the serodiagnosis of leptospirosis both in humans and in animals world health organization who 2003. Multilocus sequence typing mlst will be performed on clinical samples that have tested pcr positive. The mat is an excellent test but it also has limitations.

A single elevated mat titer in a patient with a compatible febrile illness and suspected exposure. What is the mat test for leptospirosis. Mat is considered the gold standard test for diagnosis of leptospirosis. It has unsurpassed specificity but its sensitivity is low compared to elisa igm slide agglutination test sat.

Laboratory definitive evidence for a confirmed case requires at least one of the following. The microscopic agglutination test mat is the basis of serological diagnosis and classification. Isolation of leptospires from a clinical specimen. If the level of antibodies called a titer is high enough or can be shown to be rising over time then infection is confirmed.

A 4 fold rise in mat titer between acute and convalescent sera. It uses panels of live leptospires ideally recent isolates representing the circulating serovars from the area where the patient became infected. The mat is best used as a screening test when investigating the possibility of l. Hardjo infection in groups or herds of cattle.

Microscopic agglutination test mat mat is regarded as a gold standard method for the diagnosis of leptospirosis but it may be only available in reference laboratories. Microscopic agglutination test mat for the diagnosis of leptospirosis and serotyping of leptospires. Mat has high sensitivity and detects serovar specific antibodies. 1965 and modified by cole et al.

Seroconversion with a 4 fold or larger rise in titer between acute and convalescent phase serum specimens obtained 2 weeks or more apart confirms the diagnosis. 1973 is the most widely used serological test for leptospirosis thiermann and garrett 1983. Laboratory test for diagnosis. The mat or microscopic agglutination test detects the presence of antibodies against leptospira in a dog s blood.

The mat which was originally described by galton et al. World organisation for animal health oie 2008. Korver department of biomedical research royal tropical institute meibergdreef 39 11005 az amsterdam the netherlands get pdf file. The microscopic agglutination test mat is the gold standard for sero diagnosis of leptospirosis because of its unsurpassed diagnostic specificity.